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KMID : 0882419930440010049
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Korean Journal of Medicine
1993 Volume.44 No. 1 p.49 ~ p.57
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Response of Oseoblastic Cell Line MC3Tc-E1 Cell to the Triiodothyronine
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윤현구
우정택/김진우/김영설/김광원/최영길/서광식
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Abstract
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ackground : thyroid hormone appers to stimulate both the osteoclast and the osteoblast, but is direct effect on bonee formation its not yet clear. This study was designed to investigate the effects of triiodothyronine
(T3) on
DNA synthesis, collagen protein synthesis and type I collagen transcript levels in osteoblastlike MC3T3-E1 cells.
Methods : the cells used in the present experiments were found to be PTH-responsive in
terms of cAMP formatiom. The cells were cultured in alpha-MEM supplemented with 10%
fetal bovine serum (FBS) and then changed to 0.1% FBS with various concentration
of triiodothyronine. DNA synthesis was studied by exming effects on the insorporation of
[(3)H] proline into collagenase digestible protein (CDP) and noncollagen protein (NCP). Type I
collagen mRNA expression was studied by Northern blot analysis.
Results : northern blot analysis showed the presence of 6.4 kb type I collagen transcrip in
control and T3-treated cultures. From this study. There was no significant differences in
DNA synthesis and percentage of collagen synthesized between t3-treated cells and
control. Northern blot analysis of T3-treated cells, using a type I alpha2 collagen probe,
revealed no significant changes in type I collagen transcripts.
Conclusion : In MC3T3-E1 cells, which reponded to PTH by increasig cAMP
formation, the presece of 6.4 kd type I collagen transcript was found. Thus MC3T3-E1 cells are
a useful model for the investigation of osteoblastic cells. Based on the data, T3 exerted no
significant effects on DNA, collagen synthesis and transcript levels in osteoblast like
MC3T3-E1 cells.
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KEYWORD
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